In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization
Clinics
; Clinics;67(10): 1171-1179, Oct. 2012. ilus, tab
Article
in En
| LILACS
| ID: lil-653481
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols.METHODS:
Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick test) with native and polymerized β-lactoglobulin in adult patients with an IgE-mediated allergy to plactoglobulin. In vitro humoral immunoreactivity was analyzed using immunoblotting. Cell-mediated immunoreactivity was analyzed using ex vivo challenges with native and polymerized β-lactoglobulin and monitored by leukocyte adherence inhibition tests.RESULTS:
The skin tests demonstrated that there was a significant reduction in immediate cutaneous reactivity after polymerization. Polymerization did not decrease the immunoblotting detection of s-IgE specific to β-lactoglobulin. Cell-mediated immunoreactivity, as assessed by ex vivo challenges and leukocyte adherence inhibition tests, did not exhibit significant differences between leukocytes challenged with native versus polymerized β-lactoglobulin.CONCLUSIONS:
The polymerization of β-lactoglobulin decreased in vivo allergenicity and did not decrease in vitro humoral or ex vivo cell-mediated immunoreactivity. Therefore, we conclude that inducing polymerization using transglutaminase represents a promising technique to produce suitable molecules for the purpose of designing oral/ sublingual tolerance induction protocols for the treatment of allergies.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Transglutaminases
/
Milk Hypersensitivity
/
Cysteine
/
Immune Tolerance
/
Lactoglobulins
Type of study:
Guideline
/
Observational_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
En
Journal:
Clinics
Journal subject:
MEDICINA
Year:
2012
Document type:
Article
Affiliation country:
Country of publication: